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Amylase, Serum

Amylase, Serum

Epic Mnemonic
Sunquest Mnemonic

LAB48
AMY

Category

Chemistry

Methodology

Enzymatic Colorimetric (Ethylidene-G7PNP)

Test Performance Schedule

Sunday - Saturday

Result Availability

Within 24 hours – STAT upon request

Specimen Required

Container

Gold top (SST), Red top (serum), or Green top (lithium heparin) tube
Alt: EDTA / Red

Volume

Pref. Vol.: 1.0 mL
Min. Vol.: 0.5 mL

Collection Instructions

• Routine venipuncture

• Immediately after collection, gently invert tube 5-10 times

• Clot 30 minutes

• Promptly centrifuge 

• If no gel barrier is present immediately transfer serum or plasma to a plastic tube and refrigerate

• Properly centrifuged gel barrier tube does not require transfer of serum or plasma to separate tube

Transportation Instructions

Refrigerated

Stability

• Room Temperature: 5 days
• Refrigerated: 5 days

CPT Codes

82150

Effective/Revised

03/21/2017

Clinical Significance

Assays of amylase activity in serum are of great significance in the diagnosis of diseases of the pancreas and investigating pancreatic function. The magnitude of the elevation of serum amylase activity is not related to the severity of pancreatic involvement; however, the greater the rise, the greater the probability of acute pancreatitis.

 

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