Bilirubin, Total

Bilirubin, Total

Epic Mnemonic
Sunquest Mnemonic

LAB50
TBIL

Category

Chemistry

Methodology

Colorimetric; Diazotization

Test Performance Schedule

Sunday - Saturday

Result Availability

Within 24 hours – STAT upon request

Container

Gold top (SST), Red top (serum), or Green top (lithium heparin) tube
Alt: EDTA / RED

Volume

Pref. Vol.: 1.0 mL serum or plasma
Min. Vol.:0.5 mL serum or plasma

Collection Instructions

• Routine venipuncture

• Immediately after collection, gently invert tube 5-10 times

• Avoid exposure to light

• Clot 30 minutes

• Promptly centrifuge

• If no gel barrier is present immediately transfer serum or plasma to a plastic tube and refrigerate

• Properly centrifuged gel barrier tube does not require transfer of serum or plasma to separate tube

Transportation Instructions

Refrigerated

Stability

• Room Temperature: 24 hours
• Refrigerated: 5 days

Causes for Rejection

• Prolonged exposure to light

CPT Codes

82247

Effective/Revised

03/22/2017

Measurement of the levels of bilirubin, an organic compound formed during the normal and abnormal destruction of red blood cells, is used in the diagnosis and treatment of liver, hemolytic, hematological, and metabolic disorders, including hepatitis and gall bladder blockage.

Bilirubin is formed in the reticuloendothelial system during the degradation of aged erythrocytes. The heme portion from hemoglobin and from other heme-containing proteins is removed, metabolized to bilirubin, and transported as a complex with serum albumin to the liver. In the liver, bilirubin is conjugated with glucuronic acid for solubilization and subsequent transport through the bile duct and elimination via the digestive tract.

Diseases or conditions which, through hemolytic processes, produce bilirubin faster than the liver can metabolize it, cause the levels of unconjugated (indirect) bilirubin to increase in the circulation. Liver immaturity and several other diseases in which the bilirubin conjugation mechanism is impaired cause similar elevations of circulating unconjugated bilirubin. Bile duct obstruction or damage to hepatocellular structure causes increases in the levels of both conjugated (direct) and unconjugated (indirect) bilirubin in the circulation.