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Cytogenetics Fibroblast Culture with Cryopreservation

Cytogenetics Fibroblast Culture with Cryopreservation

Alternate Test Name

Alternate Name

Epic Mnemonic
Sunquest Mnemonic

LABXXXX
CFCWC

Category

Sendouts

Methodology

Fibroblasts are cultured and suspended in 10% Dimethylsulfoxide (DMSO) freeze media for prolonged storage of viable cells in liquid nitrogen.

Test Performance Schedule

Monday - Friday

Result Availability

2 – 4 days

Specimen Required

Container

Sterile transport tube with tissue culture media

Volume

Pref. Vol.: 5.5 mm
Min. Vol.: 3.0 mm

Collection Instructions

• Place in sterile container with either tissue culture media or transport media and an antibiotic such as Pen/Strep or Gentamicin
• Tissue may be sorted overnight in medium at 4°C
• Specimens placed in formalin are not acceptable

Transportation Instructions

Room temperature

Stability

• Room Temperature: 3 days
• Refrigerated: Unacceptable
• Frozen: Unacceptable

Causes for Rejection

Specimens placed in formalin

Remarks

If ambient temperature exceeds 80°F or is below 40°F, please send in an insulated container

CPT Codes

88233, 88240

Effective/Revised

3/28/2023

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