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Direct and Indirect Antiglobulin Test (Direct and Indirect Coombs)

Direct and Indirect Antiglobulin Test (Direct and Indirect Coombs)

Mnemonic

LAB1282
DCAS

Result Availabilty

Within 24 hours - STAT upon request

Specimen Required

Container

Lavender top (EDTA) tube

Volume

Pref. Vol.: 6.0 mL whole blood
Min. Vol.: 2.0 mL whole blood

Collection Instructions

• Routine venipuncture
• Immediately after collection, gently invert tube 5-10 times
• Refrigerate entire Lavender top tube of whole blood
• Do not centrifuge or remove plasma from red cell portion of specimen

Transportation Instructions

Refrigerated

Causes for Rejection

• Gross hemolysis
• Received more than 24 hours after collection
• Collected in wrong tube type

CPT Codes

86880 (DAT)
86850 (Antibody Screen)

Effective/Revised

12/22/14

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