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Factor VIII Assay

Factor VIII Assay

Alternate Test Name

Factor VIII (8) Activity
Hemophilia A

Epic Mnemonic
Sunquest Mnemonic

LAB306
F8

Category

Sendouts

Methodology

Activated Partial Thromboplastin (APTT) clot-based assay with plasma deficient substrate

Test Performance Schedule

Monday – Friday

Result Availability

1-5 days

Specimen Required

Container

Light blue top (3.2% buffered sodium citrate) tube

Volume

Pref. Vol.: 2.7 mL (2-3 aliquots, minimum of 0.5 ml each)
Min. Vol.: 1.8 mL (2-3 aliquots, minimum of 0.5 ml each)

Collection Instructions

• Routine venipuncture
• Immediately after collection, gently invert tube 5-10 times
• Specimen must be processed within 1 hour of collection
• Platelet-poor plasma: Centrifuge light-blue top for 15 minutes at approximately 1500 g within 1 hour of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer, and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL).
• Separate plasma into 2-3 aliquots, minimum of 0.5 mL each
• Freeze immediately

Transportation Instructions

Frozen

Stability

• Room Temperature: 1 hour (whole blood state)
• Refrigerated: Unacceptable
• Frozen: 2 months

Causes for Rejection

• Citrate tube underfilled
• Clotted or hemolyzed specimens
• Platelet-poor plasma not frozen with 1 hour of collection

Remarks

Platelet-poor plasma (PPP) samples obtained by double centrifugation are critical for accurate coagulation studies as platelet contamination may cause spurious results

CPT Codes

85240

Effective/Revised

8/20/2019

 

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