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Heparin Dependent Antibody

Heparin Dependent Antibody

Alternate Test Name

Heparin Dependent Antibody Level
Heparin Induced Thrombocytopenia
Heparin PF4 Antibody

Epic Mnemonic
Sunquest Mnemonic

LAB3303
HIT

Category

Sendouts

Test Performance Schedule

Monday – Friday
Saturday and Sunday upon request

Result Availability

1 – 3 days

Specimen Required

Container

Red top (serum) tube

Volume

Pref. Vol.: 4 mL serum
Min. Vol.: 3 mL serum

Collection Instructions

• After collection, gently invert tube 8-10 times.
• Allow specimen to clot for 30 minutes and centrifuge
• Pipette serum into 3 screw-capped transport tubes (0.5 mL, 0.5 mL, and 2.0mL) and freeze.

Transportation Instructions

Frozen

Stability

After separation from cells:
• Room temperature: Unacceptable
• Refrigerated: Unacceptable
• Frozen: 2 months

Causes for Rejection

Sample collected in any collection tube other than a red top tube

Remarks

Positive or borderline results will reflex to Serotonin Release Assay

CPT Codes

86022

Effective/Revised

02/28/2020
Clinical lSignificance

Patients should be evaluated using the 4T Score before the Heparin Dependent Antibody test is ordered. Click on this link to see the 4T Score table.

A 4T Score of 3 or less is considered indicative of a low probability of heparin induced thrombocytopenia (HIT). The Heparin Dependent Antibody test should NOT be ordered in such patients. A 4T Score of 4 or higher should be considered indicative of intermediate or high probability of HIT. The Heparin Dependent Antibody test should be ordered only in patients with a 4T Score at or above this level.

 

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