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Hepatitis B Surface AG

Hepatitis B Surface Antigen

Alternate Test Name

HBsAg

HBV Surface Antigen

Epic Mnemonic
Sunquest Mnemonic

LAB471
HBSAG

Category

Chemistry

Methodology

Electrochemiluminescence immunoassay

Test Performance Schedule

Sunday - Saturday

Result Availability

Within 24 hours

Specimen Required

Container

Gold top (SST) or Red top (serum) tube
Also acceptable: PST (Lithium heparin) or Lavender top (EDTA) tube

Volume

Pref. Vol.: 1.0 mL serum
Min. Vol.: 0.5 mL serum

Collection Instructions

• Routine venipuncture

• Immediately after collection, gently invert tube 5-10 times

• Clot 30 minutes

• Promptly centrifuge 10 minutes

• If no gel barrier is present immediately transfer serum or plasma to a plastic tube and refrigerate

• Properly centrifuged gel barrier tube does not require transfer of serum or plasma to separate tube

Transportation Instructions

Refrigerated

Stability

• Refrigerated: 5 days

Remarks

Medicare Medical Necessity Policy

Biotin, also referred to as Vitamin B7 or Vitamin H, can interfere with this test when taken in mega doses of 5mg (5000 mcg) or more. It is often promoted as a skin and hair beauty aid. Please ask your patients to refrain from taking Biotin or supplements containing Biotin for at least 24 hours before collecting specimens for lab testing.

https://biotinfacts.roche.com/

https://www.fda.gov/medicaldevices/safety/alertsandnotices/ucm586505.htm

CPT Codes

86706

Effective/Revised

03/31/2017

Clinical Significance

The hepatitis B surface antigen, a polypeptide of varying size, is a component of the external envelope of the hepatitis B virus particle (HBV). The blood of persons infected with HBV contains, in addition to intact infectious HBV particles, smaller non-infectious “empty” envelope particles, which are formed in great excess and also contain the hepatitis B surface antigen.

 

The HBsAg determinant a, against which the immune response is mainly directed, is common to all HBsAg particles. The detection of HBsAg in human serum or plasma indicates an infection by the hepatitis B virus. HBsAg is the first immunological marker and is generally present some days or weeks before clinical symptoms begin to appear.

 

HBsAg is observed in persons with acute and chronic hepatitis B infections. In rare cases an HBV infection can also take place without HBsAg being detectable. HBsAg tests are used within the scope of diagnostic procedures to identify persons infected with HBV and to prevent the transmission of the hepatitis B virus.

 

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