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Hepatitis C Genotyping

Hepatitis C Genotyping

Alternate Test Name

Hepatitis C Genotype w/Amplification; Hep C Genotype

Epic Mnemonic
Sunquest Mnemonic

LAB915
HCGEN

Category

Sendouts

Methodology

Multiplex Reverse Transcription -Polymerase Chain Reaction (RT-PCR) followed by solid phase electrochemical analysis

Test Performance Schedule

Once per week

Result Availability

1 – 7 days

Specimen Required

Container

Lavender top (EDTA) or Gold top (SST) tube

Volume

Pref. Vol.: 6.0 mL whole blood (EDTA) or 5.0 mL whole blood (SST)
Min. Vol.: 2.0 mL plasma or serum

Collection Instructions

• Routine venipuncture
• Allow serum tube to clot
Promptly centrifuge 15 minutes
Immediately transfer serum or plasma to separate plastic tube within 6 hours of collection
• Freeze

Transportation Instructions

Frozen

Stability

After separation from cells:
• Room temperature: 24 hours
• Refrigerated: 3 days
• Frozen (preferred): 6 weeks

Causes for Rejection

Serum or plasma not separated from cells within 6 hours of collection

Remarks

Hepatitis C Virus RNA Quantitation by RT-PCR testing (test code #4185) must be ordered before Hepatitis C Genotyping will be performed. HCV Genotype testing is intended to be used as a follow-up procedure for patients who have been identified with an active HCV infection, with an HCV viral load adequate for HCV Genotyping (over 500 IU/mL).

CPT Codes

87521, 87902

Effective/Revised

11/19/2019

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