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Insulin, Free and Total

Insulin, Free and Total

Alternate Test Name

Insulin, Free and Total, Serum

Epic Mnemonic
Sunquest Mnemonic

LAB3014
INSFTB

Category

Sendouts

Methodology

Electrochemiluminescence Immunoassay

Test Performance Schedule

Monday - Saturday

Result Availability

1 - 2 days

Specimen Required

Container

Gold top (SST) or Red top (serum) tube

Volume

Pref. Vol.: 2.0 mL
Min. Vol.: 0.75 mL

Collection Instructions

• Routine venipuncture
• Patient to be fasting (8 hours)
• Serum samples clot 30 minutes
• Promptly centrifuge 15 minutes
• Immediately transfer serum to separate plastic tube
• Refrigerate

Transportation Instructions

• Refrigerate

Stability

After separation from cells:
• Room temperature: 24 hours
• Refrigerated: 14 days
• Frozen (preferred): 180 days

Causes for Rejection

• Grossly hemolyzed or lipemic specimens
• Autopsy/postmortem specimen

Remarks

For 12 hours before this test do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins

CPT Codes

83527 – Free Insulin
83525 – Total Insulin

Effective/Revised

10/29/2021

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