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JC Virus DNA by Quantitative RT-PCR, Plasma or Serum

JC Virus DNA by Quantitative Real-time PCR, Plasma or Serum

Alternate Test Name

JCV; JC Polyoma Virus; John Cunningham Virus

Epic Mnemonic
Sunquest Mnemonic

LAB3247
JCVQ

Category

Sendouts

Methodology

Quantitative Real-Time Polymerase Chain Reaction (PCR)

Test Performance Schedule

Monday - Saturday

Result Availability

2 – 4 days

Specimen Required

Container

Plasma: Lavender top (EDTA) or Yellow top (ACD-A or B) tube
Serum: Red top (serum) tube

Volume

Pref. Vol.: 2.0 mL plasma or serum
Min. Vol.: 0.5 mL plasma or serum

Collection Instructions

• Routine venipuncture
• Immediately after collection, gently invert tube 5-10 times
• Centrifuge 15 minutes
• Immediately transfer plasma or serum to a separate tube
• Freeze

Transportation Instructions

Frozen

Stability

• Room Temperature: Unacceptable
• Refrigerated: Unacceptable
• Frozen: 1 week

Causes for Rejection

Specimens received not frozen

CPT Code

87799

Effective/Revised

8/28/2019

Clinical Significance

JCV is the etiologic agent of progressive multifocal leukoencephalopathy (PML) which is mainly seen in HIV patients, organ transplant patients, and other immunodeficient syndromes. In addition to PML, JCV also causes nephropathy in the renal transplant setting, although with considerably less frequency than BKV. JCV should always be considered in an immunocompromised patient with progressively deteriorating CNS function. Quantitative JCV DNA PCR can be used to detect JCV in CSF in the setting of CNS disease, and blood and urine in the setting of renal dysfunction. Quantitative DNA PCR can be used to track the course of infection as well as monitor response to treatment.

 

 

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