Lupus Anticoagulant, Circulating Anticoagulant Detection

Lupus Anticoagulant, Circulating Anticoagulant Detection

Alternate Test Name

Lupus Anticoagulant Screen; Russell Viper Venom; Lupus Screen

Epic Mnemonic
Sunquest Mnemonic

LAB478
LEANTB

Category

Sendouts

Methodology

Simplified Dilute Russell Viper Venom test

Test Performance Schedule

Monday - Friday

Result Availability

1 – 4 days

Container

4 – 6 Light blue top (3.2% buffered sodium citrate) tubes

Volume

Pref. Vol.: 10.8 mL (4 - 6 aliquots, minimum of 0.5 ml each)
Min. Vol.: 8.1 mL (4 - 6 aliquots, minimum of 0.5 ml each)

Collection Instructions

• Routine venipuncture
• Immediately after collection, gently invert tube 5 -10 times
• Specimen must be processed within 1 hour of collection
• Platelet-poor plasma: Centrifuge light-blue top for 15 minutes at approximately 1500 g within 1 hour of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer, and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL).
• Separate plasma into 4-6 aliquots, minimum of 0.5 mL each
• Freeze immediately

Transportation Instructions

Frozen

Stability

• Room Temperature: Unacceptable
• Refrigerated: Unacceptable
• Frozen: 2 months (Platelet poor plasma, aliquoted and frozen within 1 hour of collection)

Causes for Rejection

• Citrate tube underfilled
• Clotted or hemolyzed specimens
• Platelet-poor plasma not frozen with 1 hour of collection

Remarks

Patient should not be on anticoagulants when being drawn for the screen.

Interfering medications include the following: Apixaban, (Eliquis®), Rivaroxaban, (Xarelto®), Dabigatran, (Pradaxa), and Argatroban. Interfering medications will lead to aberrant/uninterpretable test results. Suggest testing when patient is not on interfering medications.

CPT Codes

85613

Effective/Revised

11/23/2020

The lupus screen is an assay used for the detection of lupus anticoagulants. The test employs a simplified Dilute Russell’s Viper Venom DRVV reagent. The test may be used to investigate a prolonged activated thromboplastin time.

Lupus anticoagulants are autoantibodies targeting negatively charged phospholipids or complexes of phospholipids. They are noted in a variety of clinical conditions, including autoimmune diseases. They are considered a risk factor for thrombosis. This test may also be useful to evaluate recurrent fetal loss.