Lyme Disease Antibody, IgG, Cerebrospinal Fluid by ELISA

Lyme Disease Antibody, IgG, Cerebrospinal Fluid by ELISA

Alternate Test Name

Lyme Antibody CSF by ELISA; Lyme CNS Infection IgG with Antibody Index Reflex, Serum and Spinal Fluid

Epic Mnemonic
Sunquest Mnemonic

LAB789
CLYME

Category

Sendouts

Methodology

Enzyme-Linked Immunosorbent Assay (ELISA)

Test Performance Schedule

Monday, Wednesday, Friday

Result Availability

1 – 3 days

Container

Submitboth of the following specimens:
CSF: Spinal Fluid Collection tube or pour-over tube
Blood: Gold top (SST) tube (needs to be collected within 24 hours of the spinal fluid specimen, preferably at the same time)

Volume

CSF:
Pref. Vol.: 1.5 mL
Min. Vol.: 1.2 mL

Blood:
Pref. Vol.: 1.5 mL
Min. Vol.: 1.2 mL

Collection Instructions

• CSF collected by lumbar, ventricular or cisternal puncture into plastic sterile tubes (use tube 2, 3, or 4)
• Routine venipuncture
• Immediately after collection, gently invert tube 5-10 times
• Promptly centrifuge 15 minutes
• Refrigerate within 2 hours of collection

Transportation Instructions

Refrigerated

Stability

CSF:
• Room Temperature: Unacceptable
• Refrigerated (preferred): 11 days
• Frozen: 35 days

Serum:
• Room Temperature: Unacceptable
• Refrigerated (preferred): 11 days
• Frozen: 35 days

Causes for Rejection

• Gross hemolysis or lipemia
• Do not submit CSF from the first vial due to the possibility of blood contamination

Remarks

Both spinal fluid (CSF) and serum are required for this test
CSF and serum must be collected within 24 hours maximum of each other

CPT Codes

86618 – Lyme spinal fluid
86618 x 2 - Lyme, Serum and spinal fluid if applicable for Antibody Index
82040 - Albumin, serum if applicable for Antibody Index
82042 - Albumin, spinal fluid if applicable for Antibody Index
82784 x 2 - IgG, serum and spinal fluid if applicable for Antibody Index

Effective/Revised

8/29/2019

Lorum Ipsum Opsom