Estradiol

Estradiol

Epic Mnemonic
Sunquest Mnemonic

LAB523
ESDL

Category

Chemistry

Methodology

Electrochemiluminescence immunoassay

Test Performance Schedule

Sunday - Saturday

Result Availability

Within 24 hours

Container

Gold top (SST), Red top (serum), or Green top (lithium heparin) tube

Volume

Pref. Vol.: 1.0 mL serum or plasma
Min. Vol.: 0.5 mL serum or plasma

Collection Instructions

• Routine venipuncture

• Immediately after collection, gently invert tube 5-10 times

• Clot 30 minutes

• Promptly centrifuge 10 minutes

• If no gel barrier is present immediately transfer serum or plasma to a plastic tube and refrigerate

• Properly centrifuged gel barrier tube does not require transfer of serum or plasma to separate tube

Transportation Instructions

Refrigerated

Stability

• Room Temperature: 12 hours
• Refrigerated: 2 days

• Frozen: 6 months

Remarks

Due to the risk of cross reactivity which will falsely increase estradiol levels, this assay should not be used when monitoring patients being treated with Fulvestrant (Faslodex). The preferred methodology for patients being treated with Fulvestrant (Faslodex) is Tandem Mass Spectrometry (TMS).

Biotin, also referred to as Vitamin B7 or Vitamin H, can interfere with this test when taken in mega doses of 5mg (5000 mcg) or more. It is often promoted as a skin and hair beauty aid. Please ask your patients to refrain from taking Biotin or supplements containing Biotin for at least 24 hours before collecting specimens for lab testing.

https://biotinfacts.roche.com/

https://www.fda.gov/medicaldevices/safety/alertsandnotices/ucm586505.htm

CPT Codes

82670

Effective/Revised

03/30/2017

Estrogens are responsible for the development of the secondary female sex characteristics. Estrogens are produced primarily in the ovary (follicle, corpus luteum), but small quantities are also formed in the testes and in the adrenal cortex. During pregnancy, estrogens are mainly formed in the placenta.

The biologically most active estrogen is estradiol. This is a steroid hormone. About 98 % of estradiol is bound to transport proteins (SHBG = sex hormone binding globulin).

Estrogen secretion is biphasic during the menstrual cycle. The determination of estradiol is utilized clinically in the elucidation of fertility disorders in the hypothalamus-pituitary-gonad axis, gynecomastia, estrogen-producing ovarian and testicular tumors and in hyperplasia of the adrenal cortex. Further clinical indications are the monitoring of fertility therapy and determining the time of ovulation within the framework of in vitro fertilization (IVF).